FABP4 Polyclonal antibody

FABP4 Polyclonal Antibody for WB, IHC, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 15872-1-AP

Synonyms

fatty acid binding protein 4, adipocyte, Fatty acid binding protein 4, ALBP, AFABP, Adipocyte-type fatty acid-binding protein

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Validation Data Gallery

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  WB analysis of rat skeletal muscle using 15872-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15872-1-AP (FABP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15872-1-AP (FABP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15872-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15872-1-AP (FABP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervix using 15872-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 15872-1-AP (FABP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 15872-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 15872-1-AP (FABP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 15872-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15872-1-AP (FABP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 15872-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 15872-1-AP (FABP4 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 15872-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 15872-1-AP (FABP4 Antibody) at dilution of 1:50.

• 

  IF Staining of HUVEC using 15872-1-AP

  Immunofluorescent analysis of HUVEC cells using 15872-1-AP (FABP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse adipose using 15872-1-AP

  IF result of anti-FABP4 (15872-1-AP, 1:500) with PFA fixed mouse adipose tissue by Dr. Daniel Kopinke.

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Tested Applications

• Positive WB detected in: rat skeletal muscle tissue, human adipose tissue, mouse skeletal muscle tissue, mouse adipose tissue, mouse brown adipose tissue, mouse heart tissue, rat adipose tissue
• Positive IHC detected in: human colon tissue, human cervix tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse adipose, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

15872-1-AP targets FABP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FABP4 fusion protein Ag8631
• Full Name: fatty acid binding protein 4, adipocyte
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC003672
• Gene Symbol: FABP4
• Gene ID (NCBI): 2167
• RRID: AB_2102440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

Publications

Species	Application	Title
mouse	IF	Cell; Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging.; Authors - Marion Claudia Salzer
human	IHC	Eur Heart J; Adipocyte fatty acid binding protein in atherosclerotic plaques is associated with local vulnerability and is predictive for the occurrence of adverse cardiovascular events.; Authors - Peeters Wouter W
mouse	WB,IF	Autophagy; Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.; Authors - Jingru Bai
mouse	IF	PLoS Biol; Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes.; Authors - Noriki Fujimoto
human	WB	Int J Obes (Lond); miR-20a regulates adipocyte differentiation by targeting lysine-specific demethylase 6b and transforming growth factor-β signaling.; Authors - J Zhou
		Am J Physiol Cell Physiol; Human Adipocytes from the Subcutaneous Superficial Layer have Greater Adipogenic Potential and Lower PPAR-γ DNA Methylation Levels than Deep Layer Adipocytes.; Authors - Kentaro Kosaka

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