FABP4 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FABP4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12802-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 12802-1-AP (same clone as 12802-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12802-1-AP (FABP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brown adipose using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brown adipose using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

---

Product Information

12802-1-PBS targets FABP4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FABP4 fusion protein Ag3912
• Full Name: fatty acid binding protein 4, adipocyte
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC003672
• Gene Symbol: FABP4
• Gene ID (NCBI): 2167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.