FABP1 Monoclonal antibody, PBS Only

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  WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug FABP1 Monoclonal antibody (68227-1-Ig, Clone:1B6C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

FABP1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 68227-1-PBS

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

liver FABP, L-FABP, Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Fatty acid binding protein 1

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

68227-1-PBS targets FABP1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FABP1 fusion protein Ag32836
• Full Name: fatty acid binding protein 1, liver
• Calculated Molecular Weight: 127 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC032801
• Gene Symbol: FABP1
• Gene ID (NCBI): 2168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P07148
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).