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FABP1 Monoclonal antibody

FABP1 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B6C8

Cat no : 68227-1-Ig

Synonyms

liver FABP, L-FABP, Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Fatty acid binding protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FABP1 Monoclonal antibody (68227-1-Ig)

    WB analysis using 68227-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using FABP1 Monoclonal antibody (68227-1-Ig)

    WB analysis of pig liver using 68227-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using FABP1 Monoclonal antibody (68227-1-Ig)

    FC experiment of HepG2 using 68227-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug FABP1 Monoclonal antibody (68227-1-Ig, Clone:1B6C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inrat liver tissue, pig liver tissue, mouse liver tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68227-1-Ig targets FABP1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FABP1 fusion protein Ag32836
Full Name fatty acid binding protein 1, liver
Calculated Molecular Weight 127 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC032801
Gene Symbol FABP1
Gene ID (NCBI) 2168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).

Protocols

Product Specific Protocols
WB protocol for FABP1 antibody 68227-1-IgDownload protocol
FC protocol for FABP1 antibody 68227-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols