Exportin 4 Recombinant antibody

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  WB analysis using 84449-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84449-1-RR (XPO4 antibody) at dilution of 1:8500 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84449-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Exportin 4 Recombinant antibody (84449-1-RR, Clone:241443A5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84449-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84449-1-RR against Human Exportin 4 were performed. The affinity constant is 0.512 nM.

Exportin 4 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84449-1-RR

Clone No.241443A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

XPO4, KIAA1721, Exportin-4, Exp4, 241443A5

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells, SW480 cells, RAW 264.7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:17000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84449-1-RR targets Exportin 4 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Exportin 4 fusion protein Ag21845
• Full Name: exportin 4
• Calculated Molecular Weight: 1151 aa, 130 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC113571
• Gene Symbol: Exportin 4/XPO4
• Gene ID (NCBI): 64328
• RRID: AB_3671975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9C0E2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

exportin 4 (XPO4) maps to chromosome 13q and belongs to the importin β family which acts as a bidirectional nuclear transport receptor. Decreased XPO4 expression has been shown to associate with hepatocellular carcinoma. A genome-wide copy number variation (CNV) association study in a small cohort of Malaysian subjects identified a CNV in XPO4 to be correlated with the progression of metabolic (dysfunction) associated fatty liver disease (MAFLD). (PMID: 34388518)