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CoraLite® Plus 488-conjugated Endoglin/CD105 Monoclonal antibody

Endoglin/CD105 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D5E8

Cat no : CL488-67075

Synonyms

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1, 2D5E8

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Human liver cancer tissue using CoraLite® Plus 488-conjugated Endoglin/CD105 Monoc (CL488-67075)

    IF Staining of Human liver cancer using CL488-67075

    Immunofluorescent analysis of (4% PFA) fixed Human liver cancer tissue using CoraLite® Plus 488 Endoglin/CD105 antibody (CL488-67075, Clone: 2D5E8 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of Human liver cancer tissue using CoraLite® Plus 488-conjugated Endoglin/CD105 Monoc (CL488-67075)

    IF Staining of Human liver cancer using CL488-67075

    Immunofluorescent analysis of (4% PFA) fixed Human liver cancer tissue using CoraLite® Plus 488 Endoglin/CD105 antibody (CL488-67075, Clone: 2D5E8 ) at dilution of 1:200.

Tested Applications

Positive IF-P detected inHuman liver cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67075 targets Endoglin/CD105 in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Endoglin/CD105 fusion protein Ag27837
Full Name endoglin
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC014271
Gene Symbol CD105
Gene ID (NCBI) 2022
RRIDAB_2919417
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Endoglin/CD105 antibody CL488-67075Download protocol
Standard Protocols
Click here to view our Standard Protocols