Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98013-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (98013-2-RR, Clone: 241196D9) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98013-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (98013-2-RR, Clone: 241196D9) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98013-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98013-2-RR against Human Endoglin/CD105 were performed. The affinity constant is 0.234 nM.

Endoglin/CD105 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98013-2-RR

Clone No.241196D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD105, 241196D9, HHT1, ENG, Endoglin

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98013-2-RR targets Endoglin/CD105 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: endoglin
• Calculated Molecular Weight: 71 kDa
• GenBank Accession Number: BC014271
• Gene Symbol: CD105
• Gene ID (NCBI): 2022
• RRID: AB_3672156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P17813
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)