Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody, PBS Only

Endoglin/CD105 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241196D9

Cat no : 98013-2-PBS

Synonyms

CD105, 241196D9, HHT1, ENG, Endoglin

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Validation Data Gallery

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  FC experiment of human PBMCs using 98013-2-RR (same clone as 98013-2-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (98013-2-RR, Clone: 241196D9) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98013-2-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98013-2-RR (same clone as 98013-2-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (98013-2-RR, Clone: 241196D9) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98013-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98013-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98013-2-RR against Human Endoglin/CD105 were performed. The affinity constant is 0.234 nM.

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Product Information

The immunogen of 98013-2-PBS is Endoglin/CD105 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: endoglin
• Calculated Molecular Weight: 71 kDa
• GenBank Accession Number: BC014271
• Gene Symbol: CD105
• Gene ID (NCBI): 2022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)