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EZH2 Monoclonal antibody, PBS Only
EZH2 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, FC (Intra), Indirect ELISA
Conjugate
Unconjugated
CloneNo.
1F10A12
Cat no : 66476-1-PBS
Synonyms
Validation Data Gallery
Product Information
66476-1-PBS targets EZH2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | EZH2 fusion protein Ag16789 |
Full Name | enhancer of zeste homolog 2 (Drosophila) |
Calculated Molecular Weight | 751 aa, 86 kDa |
Observed Molecular Weight | 90-102 kDa |
GenBank Accession Number | BC010858 |
Gene Symbol | EZH2 |
Gene ID (NCBI) | 2146 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)