EXOSC6 Recombinant antibody

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  WB analysis using 84346-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84346-4-RR (EXOSC6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84346-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC6 antibody (84346-4-RR, Clone: 241535C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84346-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug EXOSC6 Recombinant antibody (84346-4-RR, Clone:241535C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84346-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84346-4-RR against Human EXOSC6 were performed. The affinity constant is below 1 pM.

EXOSC6 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84346-4-RR

Clone No.241535C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241535C3, EAP4, exosome component 6, hMtr3, hMtr3p

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, HaCat cells, HEK-293 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84346-4-RR targets EXOSC6 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EXOSC6 fusion protein Ag33513
• Full Name: exosome component 6
• Calculated Molecular Weight: 28kd
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: NM_058219
• Gene Symbol: EXOSC6
• Gene ID (NCBI): 118460
• RRID: AB_3671887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5RKV6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC6 (exosome component 6), also known as p11 or MTR3. The calculated molecular weight of EXOSC6 is 28 kDa. And it has low tissue specificity. The gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. It is also a component of the RNA exosome complex (PMID: 29906447). The composition of human exosome is similar to its yeast counterpart. EXOSC6 is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.