EXOC1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

EXOC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11690-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of rat brain using 11690-1-AP (same clone as 11690-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 11690-1-AP (same clone as 11690-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11690-1-AP (same clone as 11690-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11690-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11690-1-AP (same clone as 11690-1-PBS)

  IP result of anti-EXOC1 (IP:11690-1-AP, 3ug; Detection:11690-1-AP 1:1000) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 11690-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11690-1-AP (same clone as 11690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11690-1-AP (EXOC1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11690-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 11690-1-AP (same clone as 11690-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (11690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11690-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 11690-1-AP (same clone as 11690-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using EXOC1 antibody (11690-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11690-1-PBS in a different storage buffer formulation.

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Product Information

11690-1-PBS targets EXOC1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOC1 fusion protein Ag2303
• Full Name: exocyst complex component 1
• Calculated Molecular Weight: 894 aa, 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC020650
• Gene Symbol: EXOC1
• Gene ID (NCBI): 55763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EXOC1 (exocyst complex component 1), also known as SEC3, is a component of the exocyst complex which is essential for the targeting of exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex is an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation.Recently it has been reported that exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion, which suggests a new paradigm of exocyst involvement in polarized targeting for intercellular transfer of viral proteins and viruses.