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KD/KO Validated

EXOC1 Polyclonal antibody

Name: EXOC1 Polyclonal antibody
Brand: Proteintech
SKU: 11690-1-AP

EXOC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11690-1-AP

Synonyms

SEC3P, SEC3L1, SEC3, Exocyst complex component Sec3, Exocyst complex component 1

Validation Data Gallery

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AllWBIPIHCIF-PIF/ICC

WB analysis of rat brain using 11690-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11690-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11690-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11690-1-AP
IP result of anti-EXOC1 (IP:11690-1-AP, 3ug; Detection:11690-1-AP 1:1000) with mouse brain tissue lysate 3600ug.
IHC staining of human gliomas using 11690-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 11690-1-AP (EXOC1 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of human placenta using 11690-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (11690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of C2C12 using 11690-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using EXOC1 antibody (11690-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Cat No. 11690-1-AP

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Tested Applications

Positive WB detected in	rat brain tissue, human brain tissue, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue
Positive IF/ICC detected in	C2C12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 10 publications below
IF	See 9 publications below
IP	See 1 publications below

Product Information

11690-1-AP targets EXOC1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EXOC1 fusion protein Ag2303
Full Name	exocyst complex component 1
Calculated Molecular Weight	894 aa, 102 kDa
Observed Molecular Weight	102 kDa
GenBank Accession Number	BC020650
Gene Symbol	EXOC1
Gene ID (NCBI)	55763
RRID	AB_2231500
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOC1 (exocyst complex component 1), also known as SEC3, is a component of the exocyst complex which is essential for the targeting of exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex is an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation.Recently it has been reported that exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion, which suggests a new paradigm of exocyst involvement in polarized targeting for intercellular transfer of viral proteins and viruses.

Protocols

Product Specific Protocols
WB protocol for EXOC1 antibody 11690-1-AP	Download protocol
IHC protocol for EXOC1 antibody 11690-1-AP	Download protocol
IF protocol for EXOC1 antibody 11690-1-AP	Download protocol
IP protocol for EXOC1 antibody 11690-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature A phosphoinositide conversion mechanism for exit from endosomes. Authors - Katharina Ketel KD Validated View Article
human	WB,IF	Nat Nanotechnol Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells. Authors - Tanmoy Saha KD Validated View Article
mouse	WB	J Cell Biol The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells. Authors - Juan José Sáez View Article
mouse	IF	Elife EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Authors - Yuki Osawa View Article
human	IF	PLoS Pathog Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape. Authors - Yuen-Yan Chang View Article
human	WB,IP	Retrovirology Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation. Authors - Mukerji Joya J View Article

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EXOC1 Polyclonal antibody

EXOC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis of rat brain using 11690-1-AP

WB analysis of mouse brain using 11690-1-AP

WB analysis of human brain using 11690-1-AP

IP experiment of mouse brain using 11690-1-AP

IHC staining of human gliomas using 11690-1-AP

IF Staining of human placenta using 11690-1-AP

IF Staining of C2C12 using 11690-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A phosphoinositide conversion mechanism for exit from endosomes.

Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.

The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells.

EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice.

Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape.

Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation.