CoraLite® Plus 488-conjugated ETV4 Recombinant antibody

ETV4 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241042E10

Cat no : CL488-83891-6

Synonyms

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Validation Data Gallery

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  IF Staining of A431 using CL488-83891-6

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 ETV4 antibody (CL488-83891-6, Clone: 241042E10 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83891-6 is ETV4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ETV4 fusion protein Ag0984
• Full Name: ets variant 4
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 61-65 kDa
• GenBank Accession Number: BC007242
• Gene Symbol: ETV4
• Gene ID (NCBI): 2118
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.