ESR2 Recombinant antibody, PBS Only (Capture/Detector)

ESR2 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240412G9

Cat no : 83447-2-PBS

Synonyms

ESTRB, ERβ, ER-beta, Erb, ER BETA

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00441-3

  Sandwich ELISA standard curve of MP00441-3, Human ESR2 Recombinant Matched Antibody Pair - PBS only. 83447-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5103. 83447-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

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  Sample test of MP00441-3

  The mean ESR2 concentration was determined to be 58.05 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load.

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  Cytometric bead array standard curve of MP00441-1

  Cytometric bead array standard curve of MP00441-1, ESR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83447-2-PBS. Detection antibody: 83447-3-PBS. Standard: Ag5103. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83447-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83447-2-PBS against Human ESR2 was performed. The affinity constant is 0.675 nM.

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Product Information

83447-2-PBS targets ESR2 as part of a matched antibody pair:

MP00441-1: 83447-2-PBS capture and 83447-3-PBS detection (validated in Cytometric bead array)

MP00441-3: 83447-4-PBS capture and 83447-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ESR2 fusion protein Ag5103
• Full Name: estrogen receptor 2 (ER beta)
• Calculated Molecular Weight: 59 kDa
• GenBank Accession Number: BC024181
• Gene Symbol: ESR2
• Gene ID (NCBI): 2100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.