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CoraLite®594-conjugated ERp72 Monoclonal antibody

ERp72 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D5F3

Cat no : CL594-66365

Synonyms

Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERP72, PDIA4, Protein disulfide isomerase A4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ERp72 Monoclonal antibody (CL594-66365)

    IF Staining of HepG2 using CL594-66365

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 ERp72 antibody (CL594-66365, Clone: 1D5F3 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated ERp72 Monoclonal antibody (CL594-66365)

    IF Staining of HeLa using CL594-66365

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66365 (ERp72 antibody) at dilution of 1:100.

Tested Applications

Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66365 targets ERp72 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERp72 fusion protein Ag6873
Full Name protein disulfide isomerase family A, member 4
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC000425
Gene Symbol ERp72
Gene ID (NCBI) 9601
RRIDAB_2883569
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.

Protocols

Product Specific Protocols
IF protocol for CL594 ERp72 antibody CL594-66365Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Redox Biol

A novel role for the ROS-ATM-Chk2 axis mediated metabolic and cell cycle reprogramming in the M1 macrophage polarization

Authors - Chunlu Li
View Article