ERp57/ERp60 Monoclonal antibody, PBS Only

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  WB analysis using 66423-1-Ig (same clone as 66423-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66423-1-Ig (same clone as 66423-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66423-1-Ig (same clone as 66423-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66423-1-Ig (same clone as 66423-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66423-1-Ig (same clone as 66423-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

ERp57/ERp60 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66423-1-PBS

Clone No.3G4G7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ERp57, PDIA3, ER60, EC:5.3.4.1, Disulfide isomerase ER-60

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Product Information

66423-1-PBS targets ERp57/ERp60 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERp57/ERp60 fusion protein Ag8741
• Full Name: protein disulfide isomerase family A, member 3
• Calculated Molecular Weight: 505 aa, 57 kDa
• Observed Molecular Weight: 57 kDa, 60 kDa
• GenBank Accession Number: BC014433
• Gene Symbol: PDIA3
• Gene ID (NCBI): 2923
• RRID: AB_2881795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P30101
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.