CoraLite® Plus 488-conjugated ERP29 Monoclonal antibody

ERP29 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8B2

Cat no : CL488-67675

Synonyms

C12orf8, ERp28, ERP29, ERp31, PDI DB, PDIA9

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Validation Data Gallery

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  IF Staining of human kidney using CL488-67675

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ERP29 antibody (CL488-67675, Clone: 1H8B2 ) at dilution of 1:200.

• 

  IF Staining of human kidney using CL488-67675

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ERP29 antibody (CL488-67675, Clone: 1H8B2 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67675 targets ERP29 in IF-P applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERP29 fusion protein Ag30262
• Full Name: endoplasmic reticulum protein 29
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC101495
• Gene Symbol: ERP29
• Gene ID (NCBI): 10961
• RRID: AB_2919516
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).