CoraLite®594-conjugated ERO1L Monoclonal antibody

ERO1L Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G12E11

Cat no : CL594-67416

Synonyms

ERO1 alpha, ERO1 L, ERO1 L alpha, ERO1 like (S. cerevisiae), ERO1 like protein alpha, ERO1L, Oxidoreductin 1 L alpha

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Validation Data Gallery

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  IF Staining of human stomach cancer using CL594-67416

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using CoraLite®594 ERO1L antibody (CL594-67416, Clone: 1G12E11 ) at dilution of 1:200.

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  IF Staining of human stomach cancer using CL594-67416

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using CoraLite®594 ERO1L antibody (CL594-67416, Clone: 1G12E11 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human stomach cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67416 targets ERO1L in IF-P applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERO1L fusion protein Ag29910
• Full Name: ERO1-like (S. cerevisiae)
• Calculated Molecular Weight: 468 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC008674
• Gene Symbol: ERO1L
• Gene ID (NCBI): 30001
• RRID: AB_2920129
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.