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ERK1/2 Recombinant antibody, PBS Only

ERK1/2 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4P8

Cat no : 83533-1-PBS

Synonyms

ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERK1/2 Recombinant antibody (83533-1-RR)

    WB analysis using 83533-1-RR (same clone as 83533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83533-1-RR (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ERK1/2 Recombinant antibody (83533-1-RR)

    IF Staining of HeLa using 83533-1-RR (same clone as 83533-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ERK1/2 antibody (83533-1-RR, Clone: 4P8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

  • Affinity experiments of 83533-1-RR

    Affinity experiments of 83533-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83533-1-RR against Human ERK1/2 were performed. The affinity constant is 15.1 nM.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83533-1-PBS is ERK1/2 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ERK1/2 fusion protein Ag20350
Full Name mitogen-activated protein kinase 3
Calculated Molecular Weight 43 kDa
GenBank Accession NumberBC013992
Gene Symbol ERK1
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.