ERF Polyclonal antibody

ERF Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 26468-1-AP

Synonyms

PE2, PE 2, Ets2 repressor factor

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Validation Data Gallery

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  WB analysis using 26468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26468-1-AP (ERF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 26468-1-AP

  IP result of anti-ERF (IP:26468-1-AP, 4ug; Detection:26468-1-AP 1:2000) with A431 cells lysate 1040 ug.

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells
• Positive IP detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26468-1-AP targets ERF in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERF fusion protein Ag24189
• Full Name: Ets2 repressor factor
• Calculated Molecular Weight: 548 aa, 59 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC022231
• Gene Symbol: ERF
• Gene ID (NCBI): 2077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERF (Ets2 repressor factor) is also named as PE-2. Erf is a gene for a ubiquitously expressed Ets DNA-binding domain-containing transcriptional repressor regulated by Erk-dependent phosphorylation (PMID: 28694332, PMID: 35258130). Erf is involved in T cell maturation, acting as a positive regulator during CD4 and eventually CD8 lineage commitment, while negatively regulates the production of γδ T cells (PMID: 35258130). Erf is required for both primitive and erythromyeloid progenitor waves of hematopoietic stem cell (HSC)-independent hematopoiesis as well as for the normal function of HSCs (PMID: 28694332). ETS2 repressor factor (ERF) insufficiency causes craniosynostosis (CRS4) in humans and mice. ERF is an ETS domain transcriptional repressor regulated by Erk1/2 phosphorylation via nucleo-cytoplasmic shuttling (PMID: 37175668).