ERCC1 Monoclonal antibody

ERCC1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1H4D5

Cat no : 66275-1-Ig

Synonyms

COFS4, ERCC1, UV20

---

Validation Data Gallery

• 

  WB analysis of A431 using 66275-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66275-1-Ig (ERCC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 66275-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed A431 cells using 66275-1-Ig(ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: A431 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66275-1-Ig targets ERCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERCC1 fusion protein Ag6417
• Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC052813
• Gene Symbol: ERCC1
• Gene ID (NCBI): 2067
• RRID: AB_2881658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; PMID: 31541631; Authors - Bin Zhu