ERBB2IP Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68159-1-Ig (same clone as 68159-1-PBS)

  WB result of ERBB2IP antibody (68159-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERBB2IP transfected HeLa cells. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68159-1-Ig (same clone as 68159-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ERBB2IP antibody (68159-1-Ig, Clone: 1A8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51110-1

  Cytometric bead array standard curve of MP51110-1, ERBB2IP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68159-1-PBS. Detection antibody: 68159-2-PBS. Standard:Ag18514. Range: 0.195-100 ng/mL.

• Featured Product
• KD/KO Validated

ERBB2IP Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 68159-1-PBS

Clone No.1A8D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

LAP2, KIAA1225, ERBIN, Erbb2-interacting protein, Densin-180-like protein

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Product Information

68159-1-PBS targets ERBB2IP as part of a matched antibody pair:

MP51110-1: 68159-1-PBS capture and 68159-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERBB2IP fusion protein Ag18514
• Full Name: erbb2 interacting protein
• Calculated Molecular Weight: 1412 aa, 158 kDa
• Observed Molecular Weight: 150-180 kDa
• GenBank Accession Number: BC115012
• Gene Symbol: ERBB2IP
• Gene ID (NCBI): 55914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96RT1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)