EPO/Erythropoietin Monoclonal antibody

EPO/Erythropoietin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1A11F3

Cat no : 66975-1-Ig

Synonyms

EPO, MVCD2, erythropoietin, Epoetin, EP

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Validation Data Gallery

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  WB analysis of Recombinant protein using 66975-1-Ig

  50 ng recombinant human EPO (cat.NO. HZ-1168) was subjected to SDS-PAGE under reducing condition followed by western blot with 66975-1-Ig (EPO antibody) at dilution of 1:10000.

• 

  IHC staining of human liver using 66975-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66975-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: recombinant human EPO (cat.NO. HZ-1168)
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

66975-1-Ig targets EPO/Erythropoietin in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPO/Erythropoietin fusion protein Ag23054
• Full Name: erythropoietin
• Calculated Molecular Weight: 193 aa, 21 kDa
• GenBank Accession Number: BC093628
• Gene Symbol: EPO/Erythropoietin
• Gene ID (NCBI): 2056
• RRID: AB_2882295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Publications

Species	Application	Title
human	WB	Front Oncol; TCF11 Has a Potent Tumor-Repressing Effect Than Its Prototypic Nrf1α by Definition of Both Similar Yet Different Regulatory Profiles, With a Striking Disparity From Nrf2.; Authors - Meng Wang
rat	WB	Front Cardiovasc Med; Molecular and Cellular Response of the Myocardium (H9C2 Cells) Towards Hypoxia and HIF-1α Inhibition.; Authors - Hari Prasad Osuru
mouse	WB	Am J Physiol Renal Physiol; The hypoxia-inducible factor-α prolyl hydroxylase inhibitor FG4592 ameliorates renal fibrosis by inducing the H3K9 demethylase JMJD1A; Authors - Takeshi Ike
mouse	WB	ChemMedChem; Perfluorocarbon Nanoparticles Incorporating GB: Artificial O2 Carrier with Antioxidant Activity and Antithrombotic Effect; Authors - Wei Meng