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Biotin-conjugated EPHX2 Polyclonal antibody
EPHX2 Polyclonal Antibody for IHC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC
Conjugate
Biotin
Cat no : Biotin-10833
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
Biotin-10833 targets EPHX2 in IHC applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EPHX2 fusion protein Ag1283 |
Full Name | epoxide hydrolase 2, cytoplasmic |
Calculated Molecular Weight | 63 kDa |
Observed Molecular Weight | 63 kDa |
GenBank Accession Number | BC013874 |
Gene Symbol | EPHX2 |
Gene ID (NCBI) | 2053 |
Conjugate | Biotin |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).
Protocols
Product Specific Protocols | |
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IHC protocol for Biotin EPHX2 antibody Biotin-10833 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |