EPHB2 Recombinant antibody, PBS Only

EPHB2 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230111A5

Cat no : 83277-1-PBS

Synonyms

EK5, EC:2.7.10.1, DRT, Developmentally-regulated Eph-related tyrosine kinase, CAPB

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Validation Data Gallery

• 

  WB analysis using 83277-1-RR (same clone as 83277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83277-1-RR (EPHB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83277-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83277-1-RR (same clone as 83277-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug EPHB2 Recombinant antibody (83277-1-RR, Clone:230111A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83277-1-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 83277-1-PBS is EPHB2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EPHB2 fusion protein Ag28786
• Full Name: EPH receptor B2
• Calculated Molecular Weight: 987 aa, 108 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC018763
• Gene Symbol: EPHB2
• Gene ID (NCBI): 2048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.