EPCAM/CD326 Recombinant antibody, PBS Only

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  WB analysis using 84073-6-RR (same clone as 84073-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84073-6-RR (same clone as 84073-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EPCAM/CD326 antibody (84073-6-RR, Clone: 241243B10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

EPCAM/CD326 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84073-6-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

84073-6-PBS targets EPCAM/CD326 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: epithelial cell adhesion molecule
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: NM_002354.3
• Gene Symbol: EPCAM
• Gene ID (NCBI): 4072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P16422
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)