CoraLite®594-conjugated EPCAM/CD326 Monoclonal antibody

• Featured Product
• KD/KO Validated

EPCAM/CD326 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A2D5

Cat no : CL594-66316

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

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Validation Data Gallery

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  IF Staining of MCF-7 using CL594-66316

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 EPCAM/CD326 antibody (CL594-66316, Clone: 2A2D5 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66316 targets EPCAM/CD326 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPCAM/CD326 fusion protein Ag15393
• Full Name: epithelial cell adhesion molecule
• Calculated Molecular Weight: 314 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014785
• Gene Symbol: EPCAM
• Gene ID (NCBI): 4072
• RRID: AB_2883555
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)