EPB41L3 Recombinant antibody

EPB41L3 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230501D7

Cat no : 83275-2-RR

Synonyms

DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B

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Validation Data Gallery

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  WB analysis of U2OS using 83275-2-RR

  U2OS cells were subjected to SDS PAGE followed by western blot with 83275-2-RR (EPB41L3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of U2OS using 83275-2-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug EPB41L3 Recombinant antibody (83275-2-RR, Clone:230501D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83275-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83275-2-RR against Human EPB41L3 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83275-2-RR is EPB41L3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EPB41L3 fusion protein Ag1089
• Full Name: erythrocyte membrane protein band 4.1-like 3
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC008377
• Gene Symbol: EPB41L3
• Gene ID (NCBI): 23136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).