CoraLite® Plus 488-conjugated EOMES/TBR2 Recombinant antibody

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  IF Staining of SH-SY5Y using CL488-83945-5

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 EOMES/TBR2 antibody (CL488-83945-5, Clone: 241036D9 ) at dilution of 1:200, CL594-Phalloidin (red).

EOMES/TBR2 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-83945-5

Clone No.241036D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

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Tested Applications

• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83945-5 targets EOMES/TBR2 in applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EOMES/TBR2 fusion protein Ag27634
• Full Name: eomesodermin homolog (Xenopus laevis)
• Calculated Molecular Weight: 73 aa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_001278182
• Gene Symbol: EOMES
• Gene ID (NCBI): 8320
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95936
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EOMES/TRB2 (also named Eomesodermin/Tbr2) encode T-box transcription factor expressed highly in the intermediate progenitor stage of the developing neuron. During migration of the neurons, radial glia divide and migrate towards the surface of the cerebral cortex, there are three stages of cellular development: radial glia, intermediate progenitors, and postmitotic projection neurons. Radial glia express Pax6, while intermediate progenitor cells express Eomesodermin/Tbr2, and postmitotic projection neurons express Tbr1 (PMID:15634788; PMID:16320258). This process also called neurogenesis, and demonstrated that Eomesodermin/Tbr2 has been implicated in neurodevelopment. According to the mouse model, several articles showed that EOMES/TRB2 KO mice having proliferating cells decreased and having smaller upper cortical layers and a smaller sub ventricular zone in the brain (PMID:18940588). What's more, Eomesodermin/Tbr2 has also been found participating in immune response (PMID:20713880).