ENOPH1 Monoclonal antibody, PBS Only (Capture)

ENOPH1 Monoclonal Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

Cytometric bead array, Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

1F1E11

Cat no : 68179-1-PBS

Synonyms

MASA, Enolase-phosphatase E1, EC:3.1.3.77, E1, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase

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Validation Data Gallery

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  WB analysis using 68179-1-Ig (same clone as 68179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68179-1-Ig (ENOPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68179-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68179-1-Ig (same clone as 68179-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ENOPH1 Monoclonal antibody (68179-1-Ig, Clone:1F1E11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68179-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50127-1

  Cytometric bead array standard curve of MP50127-1, ENOPH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68179-1-PBS. Detection antibody: 68179-2-PBS. Standard:Ag12553. Range: 0.781-100 ng/mL.

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Product Information

68179-1-PBS targets ENOPH1 as part of a matched antibody pair:

MP50127-1: 68179-1-PBS capture and 68179-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ENOPH1 fusion protein Ag12553
• Full Name: enolase-phosphatase 1
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29-35 kDa
• GenBank Accession Number: BC065815
• Gene Symbol: ENOPH1
• Gene ID (NCBI): 58478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.