Anti-Human EMR2/CD312 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 98260-1-RR (same clone as 98260-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98260-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98260-1-RR (same clone as 98260-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11)(red) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98260-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98260-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98260-1-RR against Human EMR2/CD312 were performed. The affinity constant is 0.529 nM.

EMR2/CD312 {ptg:Brand} Recombinant Antibody for FC

Cat No. 98260-1-PBS

Clone No.242042B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

EMR2, EGF-like module receptor 2, EGF like module receptor 2, CD312, Adhesion G protein-coupled receptor E2

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Product Information

98260-1-PBS targets EMR2/CD312 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: egf-like module containing, mucin-like, hormone receptor-like 2
• Calculated Molecular Weight: 90 kDa
• GenBank Accession Number: NM_013447.3
• Gene Symbol: EMR2
• Gene ID (NCBI): 30817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UHX3-1
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as CD312 or ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing (PMID: 10903844). EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation (PMID: 31969668).The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation (PMID: 27905560).