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WB analysis of human colon using 60047-1-Ig (same clone as 60047-1-PBS)
Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
WB analysis of human colon using 60047-1-Ig (same clone as 60047-1-PBS)
human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
WB analysis of human rectum using 60047-1-Ig (same clone as 60047-1-PBS)
human rectum tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60047-1-Ig (same clone as 60047-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.
IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

EMILIN1 Monoclonal antibody, PBS Only

EMILIN1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 60047-1-PBS

Clone No.2A5C8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

EMI, EMILIN, EMILIN 1, EMILIN1, gp115

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60047-1-PBS targets EMILIN1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	EMILIN1 fusion protein Ag1016 Predict reactive species
Full Name	elastin microfibril interfacer 1
Calculated Molecular Weight	107 kDa
Observed Molecular Weight	120-130 kDa
GenBank Accession Number	BC007530
Gene Symbol	EMILIN1
Gene ID (NCBI)	11117
RRID	AB_2100191
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9Y6C2
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).