EMILIN1 Monoclonal antibody

EMILIN1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A5C8

Cat no : 60047-1-Ig

Synonyms

EMI, EMILIN, EMILIN 1, EMILIN1, gp115



Tested Applications

Positive WB detected inhuman colon tissue, human rectum tissue
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue, human kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman ovary tumor tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60047-1-Ig targets EMILIN1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EMILIN1 fusion protein Ag1016
Full Name elastin microfibril interfacer 1
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC007530
Gene Symbol EMILIN1
Gene ID (NCBI) 11117
RRIDAB_2100191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).

Protocols

Product Specific Protocols
WB protocol for EMILIN1 antibody 60047-1-IgDownload protocol
IHC protocol for EMILIN1 antibody 60047-1-IgDownload protocol
IF protocol for EMILIN1 antibody 60047-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Connect Tissue Res

EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro.

Authors - Nakatomi Yuka Y
humanIHC

Hernia

Elastic fibers in the anterior abdominal wall.

Authors - Fachinelli A A