EMAP II Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

EMAP II Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11091-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of A549 using 11091-1-AP (same clone as 11091-1-PBS)

  WB result of EMAP II antibody (11091-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMAP II transfected A549 cells. This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11091-1-AP (same clone as 11091-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11091-1-AP (EMAP II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

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  IP experiment of SGC-7901 using 11091-1-AP (same clone as 11091-1-PBS)

  IP result of anti-EMAP II (IP:11091-1-AP, 4ug; Detection:11091-1-AP 1:500) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 11091-1-AP (same clone as 11091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11091-1-AP (EMAP II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 11091-1-AP (same clone as 11091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11091-1-AP (EMAP II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11091-1-AP (same clone as 11091-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EMAP II antibody (11091-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

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Product Information

11091-1-PBS targets EMAP II in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EMAP II fusion protein Ag1560
• Full Name: small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa and 68 kDa
• GenBank Accession Number: BC014051
• Gene Symbol: EMAP II
• Gene ID (NCBI): 9255
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).