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- KD/KO Validated
EMAP II Polyclonal antibody
EMAP II Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11091-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, SGC-7901 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue |
| Positive IP detected in | SGC-7901 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
11091-1-AP targets EMAP II in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EMAP II fusion protein Ag1560 |
| Full Name | small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34 kDa and 68 kDa |
| GenBank Accession Number | BC014051 |
| Gene Symbol | EMAP II |
| Gene ID (NCBI) | 9255 |
| RRID | AB_2182590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EMAP II antibody 11091-1-AP | Download protocol |
| IHC protocol for EMAP II antibody 11091-1-AP | Download protocol |
| IF protocol for EMAP II antibody 11091-1-AP | Download protocol |
| IP protocol for EMAP II antibody 11091-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis. | ||
J Biol Chem Structural control of caspase-generated glutamyl-tRNA synthetase by appended noncatalytic WHEP domains. | ||
Proteomics Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma. | ||
Int J Mol Med Changes in the expression of endothelial monocyte‑activating polypeptide II in the rat hippocampus following status epilepticus. | ||
Cancer Commun (Lond) AIMP1 promotes multiple myeloma malignancy through interacting with ANP32A to mediate histone H3 acetylation | ||
Proc Natl Acad Sci U S A Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Krishnendu (Verified Customer) (02-27-2020) | Very strong antibody and works nicely for both WB and IP.
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