ELAVL2 Monoclonal antibody, PBS Only

ELAVL2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8C10

Cat no : 67097-1-PBS

Synonyms

ELAV like neuronal protein 1, ELAV like protein 2, ELAV2, ELAVL2, HEL N1, HELN1, Hu antigen B, HUB

---

Validation Data Gallery

• 

  WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67097-1-Ig (same clone as 67097-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67097-1-Ig (same clone as 67097-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67097-1-Ig (same clone as 67097-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67097-1-Ig (same clone as 67097-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 67097-1-Ig (same clone as 67097-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67097-1-Ig (same clone as 67097-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67097-1-PBS targets ELAVL2 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ELAVL2 fusion protein Ag28310
• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC030692
• Gene Symbol: ELAVL2
• Gene ID (NCBI): 1993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].