Neutrophil Elastase Polyclonal antibody, PBS Only

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  WB analysis using 27642-1-AP (same clone as 27642-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 27642-1-AP (ELA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 27642-1-AP (same clone as 27642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 27642-1-AP (same clone as 27642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 27642-1-AP (same clone as 27642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 27642-1-AP (same clone as 27642-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ELA2 antibody (27642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

Neutrophil Elastase Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 27642-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ELANE, ELA2, HNE, HLE, Elastase 2

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Product Information

27642-1-PBS targets Neutrophil Elastase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neutrophil Elastase fusion protein Ag26634
• Full Name: elastase 2, neutrophil
• Calculated Molecular Weight: 267 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC074816
• Gene Symbol: ELA2
• Gene ID (NCBI): 1991
• RRID: AB_2918126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08246
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.