Neutrophil Elastase Polyclonal antibody

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  WB analysis using 27642-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 27642-1-AP (ELA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 27642-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27642-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 27642-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 27642-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ELA2 antibody (27642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Neutrophil Elastase Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27642-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ELANE, ELA2, HNE, HLE, Elastase 2

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Tested Applications

• Positive WB detected in: THP-1 cells, U-937 cells
• Positive IHC detected in: human lymphoma tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

27642-1-AP targets Neutrophil Elastase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neutrophil Elastase fusion protein Ag26634
• Full Name: elastase 2, neutrophil
• Calculated Molecular Weight: 267 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC074816
• Gene Symbol: ELA2
• Gene ID (NCBI): 1991
• RRID: AB_2918126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08246
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Mol Biol Lett; Obacunone alleviates ferroptosis during lipopolysaccharide-induced acute lung injury by upregulating Nrf2-dependent antioxidant responses.; Authors - Jin Li
bovine	IF	Animals (Basel); Effects of Hormone, NEFA and SCFA on the Migration of Neutrophils and the Formation of Neutrophil Extracellular Traps in Dairy Cows.; Authors - Guanxin Lv
mouse	WB	Cancer Immunol Res; Tumor cell-derived microparticles induced by methotrexate augment T-cell antitumor responses by down-regulating expression of PD-1 in neutrophils; Authors - Pingwei Xu
rat	IHC	Front Pharmacol; RNA-sequencing approach for exploring the protective mechanisms of dexmedetomidine on pancreatic injury in severe acute pancreatitis; Authors - Jiaqi Yao
rat	WB	Int Immunopharmacol; Neutrophil extracellular traps and neutrophil extracellular traps-related genes are involved in new-onset atrial fibrillation in LPS-induced sepsis; Authors - Jie Xiang
human	WB	bioRxiv; Extracellular Vesicle-Associated Neutrophil Elastase Activates Hepatic Stellate Cells and Promotes Liver Fibrogenesis via ERK1/2 Pathway; Authors - Regina Oshins