EIF5A Monoclonal antibody, PBS Only

EIF5A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D1B6

Cat no : 67214-1-PBS

Synonyms

eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A, eIF 4D

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Validation Data Gallery

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  WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67214-1-Ig (same clone as 67214-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF5A antibody (67214-1-Ig, Clone: 2D1B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

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Product Information

67214-1-PBS targets EIF5A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF5A fusion protein Ag28588
• Full Name: eukaryotic translation initiation factor 5A
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC001832
• Gene Symbol: EIF5A
• Gene ID (NCBI): 1984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.