EIF5A Monoclonal antibody

EIF5A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D1B6

Cat no : 67214-1-Ig

Synonyms

eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A, eIF 4D

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Validation Data Gallery

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  WB analysis using 67214-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67214-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67214-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67214-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 67214-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF5A antibody (67214-1-Ig, Clone: 2D1B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, LNCaP cells, Jurkat cells, NIH/3T3 cells, HEK-293 cells, PC-3 cells, HSC-T6 cells, RAW 264.7 cells, HaCaT cells, HepG2 cells, SK-BR-3 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67214-1-Ig targets EIF5A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF5A fusion protein Ag28588
• Full Name: eukaryotic translation initiation factor 5A
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC001832
• Gene Symbol: EIF5A
• Gene ID (NCBI): 1984
• RRID: AB_2882506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.