EIF4E2 Polyclonal antibody, PBS Only

EIF4E2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12227-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 12227-1-AP (same clone as 12227-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12227-1-AP (same clone as 12227-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12227-1-AP (same clone as 12227-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 12227-1-AP (same clone as 12227-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12227-1-AP (same clone as 12227-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 12227-1-AP (same clone as 12227-1-PBS)

  IP result of anti-EIF4E2 (IP:12227-1-AP, 4ug; Detection:12227-1-AP 1:500) with MCF-7 cells lysate 1280ug. This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12227-1-AP (same clone as 12227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12227-1-AP (same clone as 12227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12227-1-AP (same clone as 12227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12227-1-AP (same clone as 12227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12227-1-PBS in a different storage buffer formulation.

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Product Information

12227-1-PBS targets EIF4E2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF4E2 fusion protein Ag2864
• Full Name: eukaryotic translation initiation factor 4E family member 2
• Calculated Molecular Weight: 245 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC021690
• Gene Symbol: EIF4E2
• Gene ID (NCBI): 9470
• RRID: AB_10642945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.