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CoraLite®594-conjugated EIF4A2 Monoclonal antibody

EIF4A2 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D11E11

Cat no : CL594-67799

Synonyms

BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®594-conjugated EIF4A2 Monoclonal antibody (CL594-67799)

    IF Staining of HEK-293 using CL594-67799

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 EIF4A2 antibody (CL594-67799, Clone: 2D11E11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®594-conjugated EIF4A2 Monoclonal antibody (CL594-67799)

    FC experiment of HEK-293 using CL594-67799

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EIF4A2 (CL594-67799, Clone:2D11E11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67799 targets EIF4A2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EIF4A2 fusion protein Ag9085
Full Name eukaryotic translation initiation factor 4A, isoform 2
Calculated Molecular Weight 408 aa, 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC012547
Gene Symbol EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2920186
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

Protocols

Product Specific Protocols
IF protocol for CL594 EIF4A2 antibody CL594-67799Download protocol
FC protocol for CL594 EIF4A2 antibody CL594-67799Download protocol
Standard Protocols
Click here to view our Standard Protocols