EIF3D Monoclonal antibody, PBS Only

EIF3D Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C5A3

Cat no : 66024-1-PBS

Synonyms

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7

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Validation Data Gallery

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  WB analysis of HepG2 using 66024-1-Ig (same clone as 66024-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 66024-1-Ig (same clone as 66024-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66024-1-Ig (same clone as 66024-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66024-1-Ig (same clone as 66024-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66024-1-PBS targets EIF3D in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF3D fusion protein Ag18092
• Full Name: eukaryotic translation initiation factor 3, subunit D
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC000328
• Gene Symbol: EIF3D
• Gene ID (NCBI): 8664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.