EIF3A Monoclonal antibody, PBS Only

EIF3A Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C7B4

Cat no : 67713-1-PBS

Synonyms

eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32

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Validation Data Gallery

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  WB analysis using 67713-1-Ig (same clone as 67713-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67713-1-Ig (EIF3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67713-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67713-1-Ig (same clone as 67713-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67713-1-Ig (EIF3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67713-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67713-1-Ig (same clone as 67713-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67713-1-Ig (EIF3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67713-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67713-1-Ig (same clone as 67713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67713-1-Ig (EIF3A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67713-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67713-1-Ig (same clone as 67713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67713-1-Ig (EIF3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67713-1-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 67713-1-Ig (same clone as 67713-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed L02 cells using EIF3A antibody (67713-1-Ig, Clone: 1C7B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67713-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67713-1-PBS targets EIF3A in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF3A fusion protein Ag24762
• Full Name: eukaryotic translation initiation factor 3, subunit A
• Calculated Molecular Weight: 1382 aa, 166 kDa
• Observed Molecular Weight: 166 kDa
• GenBank Accession Number: BC114429
• Gene Symbol: EIF3A
• Gene ID (NCBI): 8661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.