EIF3A Monoclonal antibody

EIF3A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1C7B4

Cat no : 67713-1-Ig

Synonyms

eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32

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Validation Data Gallery

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  WB analysis using 67713-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67713-1-Ig (EIF3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67713-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67713-1-Ig (EIF3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67713-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67713-1-Ig (EIF3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67713-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67713-1-Ig (EIF3A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67713-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67713-1-Ig (EIF3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 67713-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed L02 cells using EIF3A antibody (67713-1-Ig, Clone: 1C7B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, K-562 cells, HeLa cell
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

67713-1-Ig targets EIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF3A fusion protein Ag24762
• Full Name: eukaryotic translation initiation factor 3, subunit A
• Calculated Molecular Weight: 1382 aa, 166 kDa
• Observed Molecular Weight: 166 kDa
• GenBank Accession Number: BC114429
• Gene Symbol: EIF3A
• Gene ID (NCBI): 8661
• RRID: AB_2882903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Biomaterials; Patient-driven discovery of CCN1 to rescue cutaneous wound healing in diabetes via the intracellular EIF3A/CCN1/ATG7 signaling by nanoparticle-enabled delivery; Authors - Jing-Si Jiang
human	WB,IF	Cell Prolif; G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration; Authors - Shuai Li
human	WB	Cell Death Differ; Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression; Authors - Yanan Pi