Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 82936-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 82936-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 82936-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 82936-1-RR
IP result of anti-EIF2S1 (IP:82936-1-RR, 4ug; Detection:82936-1-RR 1:3000) with HepG2 cells lysate 1470 ug.
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2S1 antibody (82936-1-RR, Clone: 230245A12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of MCF-7 using 82936-1-RR
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of HeLa using 82936-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 82936-1-RR
Biolayer interferometry (BLl) kinetic assays of 82936-1-RR against Human EIF2S1 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HeLa cells, MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
82936-1-RR targets EIF2S1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 82936-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 82936-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 82936-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 82936-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82936-1-RR (EIF2S1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 82936-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82936-1-RR (EIF2S1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HepG2 using 82936-1-RR
IP result of anti-EIF2S1 (IP:82936-1-RR, 4ug; Detection:82936-1-RR 1:3000) with HepG2 cells lysate 1470 ug.
IF/ICC Figures
IF Staining of HepG2 using 82936-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2S1 antibody (82936-1-RR, Clone: 230245A12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC (INTRA) Figures
FC experiment of MCF-7 using 82936-1-RR
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of HeLa using 82936-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 82936-1-RR
Biolayer interferometry (BLl) kinetic assays of 82936-1-RR against Human EIF2S1 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
EIF2S1 Recombinant antibody
Catalog Number
82936-1-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:250-1:1000 IF/ICC : 1:500-1:2000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
