EIF2S1 Recombinant antibody, PBS Only

EIF2S1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230245A12

Cat no : 82936-1-PBS

Synonyms

EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A, eIF 2 alpha

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Validation Data Gallery

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  WB analysis using 82936-1-RR (same clone as 82936-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82936-1-RR (same clone as 82936-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82936-1-RR (same clone as 82936-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82936-1-RR (same clone as 82936-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 82936-1-RR (same clone as 82936-1-PBS)

  IP result of anti-EIF2S1 (IP:82936-1-RR, 4ug; Detection:82936-1-RR 1:3000) with HepG2 cells lysate 1470 ug. This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82936-1-RR (same clone as 82936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82936-1-RR (EIF2S1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82936-1-RR (same clone as 82936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82936-1-RR (EIF2S1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82936-1-RR (same clone as 82936-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2S1 antibody (82936-1-RR, Clone: 230245A12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 82936-1-RR (same clone as 82936-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82936-1-RR (same clone as 82936-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82936-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82936-1-RR against Human EIF2S1 were performed. The affinity constant is below 1 pM.

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Product Information

82936-1-PBS targets EIF2S1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EIF2S1 fusion protein Ag1645
• Full Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC002513
• Gene Symbol: EIF2S1
• Gene ID (NCBI): 1965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.