CoraLite® Plus 488-conjugated EIF2AK2, PKR Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF2AK2, PKR Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18244

Synonyms

eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated

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Validation Data Gallery

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  IF Staining of HeLa using CL488-18244

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EIF2AK2, PKR antibody (CL488-18244) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18244 targets EIF2AK2, PKR in IF/ICC applications and shows reactivity with human, mouse , rat samples.

• Tested Reactivity: human, mouse , rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF2AK2, PKR fusion protein Ag13010
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 2
• Calculated Molecular Weight: 551 aa, 62 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC093676
• Gene Symbol: EIF2AK2
• Gene ID (NCBI): 5610
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.