EIF1AX Polyclonal antibody, PBS Only

EIF1AX Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14654-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14654-1-AP (same clone as 14654-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 14654-1-AP (same clone as 14654-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF1AX antibody (14654-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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Product Information

14654-1-PBS targets EIF1AX in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF1AX fusion protein Ag6253
• Full Name: eukaryotic translation initiation factor 1A, X-linked
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-23 kDa
• GenBank Accession Number: BC067851
• Gene Symbol: EIF1AX
• Gene ID (NCBI): 1964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.