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EIF1AX Polyclonal antibody

EIF1AX Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14654-1-AP

Synonyms

eIF-1A X isoform, eIF1A X isoform, EIF1A, eIF 4C, eIF 1A X isoform

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF1AX Polyclonal antibody (14654-1-AP)

    WB analysis using 14654-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HepG2 cells using EIF1AX Polyclonal antibody (14654-1-AP)

    WB analysis of HepG2 using 14654-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EIF1AX Polyclonal antibody (14654-1-AP)

    WB analysis of HepG2 using 14654-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using EIF1AX Polyclonal antibody (14654-1-AP)

    WB analysis of A375 using 14654-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF1AX Polyclonal antibody (14654-1-AP)

    IF Staining of HepG2 using 14654-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF1AX antibody (14654-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inHepG2 cells, A375 cells, HeLa cells, PC-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14654-1-AP targets EIF1AX in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF1AX fusion protein Ag6253
Full Name eukaryotic translation initiation factor 1A, X-linked
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-23 kDa
GenBank Accession NumberBC067851
Gene Symbol EIF1AX
Gene ID (NCBI) 1964
RRIDAB_2878072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.

Protocols

Product Specific Protocols
WB protocol for EIF1AX antibody 14654-1-APDownload protocol
IF protocol for EIF1AX antibody 14654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics

Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.

Authors - Hao-Yu Li
View Article